In the present study we carry out the gas gun experiment and the numerical simulation to investigate the lethal effect of shock waves to a marine microorganism and behavior of waves in target containers. The marine Vibrio sp. that is isolated from ship's ballast water is used. After the impact, Vibrio sp. is cultivated for 24 hours at 30 degrees. The shock pressure effect is judged by the number of colony in agar plates. The pressure in a container is measured by the piezo-electric film gauge. We can get 2.5 kbars shock pressure in a small type container and realize 99.99% of lethal rate. On the other hand, the pressure propagation phenomena in the container are calculated by the LS-DYNA. From the comparison between experimental result and numerical one we examine the generation process of high pressure in the container.