Abstract
In bio-engineering field, the micro-scale systems, such as μTAS, attract our interest for the purpose of the improvement of efficiency of bio-chemical analysis and miniaturization of the equipment by integrating micro fluidics elements on a micro-chip. However, mixing reagent solutions each other is difficult in those systems because of extremely low Reynolds number. Moreover, a DNA sample in the solution behaves in the nano-scale, which is smaller than micro-scale, so that it is difficult to control both reagent concentration and DNA behavior properly. In this study, we calculated the electroosmotic flow with reversed flow in micro-channel by means of 2D NS equations and evaluated the effect of the reversed flow on the diffusion phenomena, and mixing condition of a DNA sample into the solution is numerically predicted by modeling the DNA with MD method and coupling the model with the calculated flow.
