Fluctuation in the measurement result of nano-particles is a common problem with recent available instruments. Therefore thermal conditioning of the sampling gas before measurement using the so-called Thermo-Conditioner was proposed in order to restrain the fluctuation in nano-particle measurement. The prime objective of this thermo-conditioner is to vaporize the volatile fraction by re-heating the diluted gas to a certain temperature and cooling down again to room temperature. As the result measurement fluctuation due to volatile fractions can be avoided. However, it is a newly developed device and the performance of this device is yet to be understood sufficiently. In this study we have attempted to clarify the effect of thermo-conditioner on the nano-particle measurement. It was found that the nuclei-mode particles are significantly influenced by the thermal conditioning temperature. Thermal conditioning at a temperature of over 300℃ was found to be optimum. Thermo-conditioner can completely vaporize the volatile fraction formed due to cold dilution in the full dilution tunnel. But it cannot vaporize all the nuclei-mode particles those exist in the raw exhaust gas.