A series calculation methodology from injector nozzle flow to in-cylinder mixture formation in diesel engine was developed and evaluated in this study. In this approach, an Eulerian three-fluid model was used to calculate cavitating nozzle flow inside the injector, and spatial distributions of velocity, turbulent energy, void fraction, etc. at the nozzle orifice exit were outputted. These output data were transferred to in-cylinder spray calculation, in which a primary break-up model was applied to the Lagrangian Discrete Droplet Model. The calculation results of high speed / high load condition were compared with the in-cylinder observation results. It was confirmed that this series calculation approach showed better good agreement on spray penetration and shape of fuel mixture.