Effect of flame stretch on laminar burning velocity was investigated by calculating outwardly propagating process of spherical flame. When flame is stretched, the flame temperature is different from adiabatic flame temperature. That causes the change of burning velocity from that of unstretched planar flame. It was confirmed that this relation was expressed by using Karlovitz number and Markstein number. Further analysis indicated that Markstein number did not depend on temperature and pressure, but equivalence ratio. In case of n-C_7H_<16>, C_3H_8, Markstein number of rich mixture is fairly lower than that of lean mixture, while it is slightly higher in case of CH_4.