Observation of adsorbed protein film on artificial cartilage by Fluorescence Staining
Details
PVA hydrogel is a kind of prospective material as artificial cartilage. It is necessary to investigate the wear properties of PVA hydrogel in severe condition. Lubricant containing protein was used in reciprocating test. Proteins formed the boundary film on PVA hydrogel, and the wear was reduced. Hyaluronate solution as lubricant contained albumin and γ-globulin which was labeled fluorescently as typical protein in synovial fluid. The remaining film was observed under amicroscopy fluorescently. Proteins formed adsorbed film on glass plate and two type of layer was observed. First layer is rich in albumin and second layer is rich in γ-globulin. There is difference between albumin and γ-globulin on adsorption strength. It is considered that albumin layer takes charge of shear, and γ-globulin layer protects material from wear. Therefore existence of layer containing albumin or γ-globulin is effective to reduce wear.
