In vitro Experiments on Behavior and Deformability of Red Blood Cell Using MicroChannel Array.
Details
In a human body, erythrocytes deform highly and can smoothly pass capillaries which diameters are narrower than cells', Since capillaries connect all organs in a body, decrease in erythrocytes deformability causes several diseases. So it is very important to evaluate the dynamic deformability of the erythrocyte on a micfocirculation level. In this research, we produced the micro channel array with a 5umx5um square cross section of 100μm in length on the silicone chip to simulate capillaries, and made experiments to estimate the deformation of erythrocyte which flows channels. After whole blood was centrifuged erythrocytes from plasma, the erythrocytes were diluted with ACD-solution or plasma by a hematocrit of 10%. Each suspension was made to flow through the micro channels on the day of drawing blood and 2 days later. As a result, it is found that an erythrocyte whose shape looked like a parachute did not deform the whole body but only the rear side of the cell
