Abstract
Vascularization is an essential phenomenon for implanted cells to proliferate, and "differentiate. We developed a sheet-like bone graft model composed of human bone marrow stromal cells (HMSCs), and beta tricalcium phosphate (beta TCP) porous beads. By combining multilayered cell-bead sheets and collagen gel, we fabricated bone implants having the space for angiogenesis, and subcutaneously implanted them in nude mice. From the result of implantation, neovascularization reaching to the central area of the graft was recognized. Osteogenesis was also observed near the surface of graft. Therefore, the newly designed implant seemed to have a high osteoconductive activity. This result would suggest a new style of bone graft, which is designed on the premise of vascularization.
