This paper presents a novel temperature measurement method for microfluidic devices; "2-Color Laser-induced Fluorescence (2-Color LIF)". In general, a conventional method (1-Color LIF) strongly depends on the excitation intensity distribution which is generated by spatio-temporal intensity fluctuation of light itself and configuration of microchannel. In contrast, the proposed method can eliminate the undesirable influence using a ratio-metric technique with two fluorescence images, because fluorescence intensity can be approximately a linear function of the excitation light intensity. Therefore, it is possible to measure the temperature distribution quantitatively and simplify temperature calibration. As a result, spatial intensity error can be reduced to about 95% as compared with 1-Color LIF image. The temperature distribution in microchannel due to heat-diflusion was visualized wilh high resolution 0.24μm×0.24μm and sensitivity 1.48%/K.
