Painful and crippling knee conditions are completely released by total knee arthroplasty (TKA). However, wear of polyethylene insert frequently shortens the life span of TKA. Because knee movement involves a complicated combination of translations and rotations. Therefore, adequate motion analysis is necessary for estimating the wear of inserts and improving the design of artificial knee joint. This study was aimed to develop a three dimensional motion analysis for artificial knee joint with high precision by using image matching technique with high-resolutional flat panel detector (FPD). The image matching was to overlap the actual FPD image and the reconstructed image. In this report, we analyzed knee prostheses during walking motion using this motion analysis method. It was possible to reproduce the in vivo relation between a femoral component and polyethylene insert. Additionaly, motion analysis of artificial knee implants could clarify typical motion pattern depending on patients.