We examined how the deformability of the particle influenced on the adhesion of particle to vWf surface, the concentration profile in flow channel and the dispersion coefficient in vitro for developing the artificial platelet substitute. We employed recombinant GlycoproteinIbα conjugated Latex Beads (rGPIbα-LB) and rGPIbα conjugated phospholipid vesicles (rGPIbα-vesicles) as platelet substitute. These particles were observed at high wall shear rate (1500 s^<-1>) with a 40% hematocrit in rectangular flow channel (height and width were 200μm), which had von Willebrand factor (vWf) surface. The rGPIbα-LB adhered to vWf surface firmly, but rGPIbα-vesicle repeated attaching to vWf surface and detaching from vWf surface. The concentration-peak of rGPIbα-LB was near vWf surface more than that of rGPIbα-vesicle. This result shows that rGPIbα-LB existed near vWf wall more than rGPIbα-vesicle. It is considered that this characteristic makes rGPIbα-LB easier to attach to vWf surface than rGPIbα-vesicle. The mobility of rGPIbα-vesicle is bigger than rGPIbα-LB in blood flow, because the dispersion coefficient of rGPIbα-vesicle was bigger than that of rGPIbα-LB. If the particle conjugated only rGPIbα-LB or AMS (rigid particle) is better than vesicle (particle with deformability) from viewpoint of adhesion and concentration profile. But the mobility of rGPIbα-vesicle is bigger than rGPIbα-LB in blood flow. We must consider which carrier we employ based on those characteristics.
