An artificial active transportation system was constructed by using two funnel type glass tubes separated by an enzyme diaphragm membrane, in which catalase was immobilized onto the single side of the dialysis membrane. By applying hydrogen peroxide (H2O2) solution into the non-enzyme side of the glass tube, the tube pressure at the enzyme side increased continuously. In consequence, an active transportation of hydrogen peroxide through the asymmetric enzyme membrane could induce the pressure increase in the enzyme side tube (max.: 5000 Pa), thus resulting in the discharge of buffer solution from the enzyme-side tube with non-pulsating flow with good repeatability (4.31% of correlation of variation). The tube pressure at the membrane side indicated the linear relationship with the concentration of hydrogen peroxide from 11.8 to 123.6 (mmol/l).
