The experimental investigation on the controlling of operation direction of a liquid crystalline actuator with orientation layer patterning has been achieved. Opposite rubbing treatment are given at the left and right side of the glass plates. When the electric field is imposed on the left (right) side of the liquid crystal cell, the liquid crystalline flow toward the right (left) side of the liquid crystal cell is induced. The movement of a particle mixed in the liquid crystal is successfully controlled by imposition of the electric pulse on the left or right side of the cell.