The velocity profile of a dilute polymer solution near the wall surface in a microchannel was clarified using evanescent wave illumination and a particle tracking velocimetry system. Fluorescent particles with a diameter of 100nm were used as tracer particles. The test fluids were polyethylene-oxide (Peo15) solutions at 5 ppm and distilled water. It was clarified that the velocity profile of the dilute polymer solution decreases significantly compared with that of water within 200nm of the wall surface. Brownian motion of the particle near the wall surface was suppressed by the polymer solution, and the suppression was emphasized under the flow condition.