Changes in diffusion of spray particles in turbulent flow was investigated by means of spray 2D visualized images. Time sequence of Probability Density Functions (PDFs) of light intensity is used to investigate diffusion of spray. Each PDFs are obtained from light intensity distributions in time sequence of spray images. Spray is injected into turbulent flow, which is generated in the wind tunnel. As a result, it is showed that diffusion of spray is expressed with a concise procedure, in which measurement instrument doesn't need to resolve each particles in a spray image. And it is observed that the spray diffusion is enhanced associated with an increase in turbulent intensity.