Living environment is complicated and varied from hour to hour, it is difficult to define a path of mobile robot. So we developed and demonstrated navigation interface for mobile robots with 3D drawing system, which user draws virtual line as a path of mobile robot in the real world. In this system user enable to navigate multiple robots by assigning color of drawing line. Moreover user can control movement speed of robot by drawing speed. As a result we can navigate mobile robots easily in living environment with obstacles.