Strength of tooth root in flexible spline (FS) in Strain Wave Gearing (SWG) becomes a problem so that FS has a very thin rim. Because teeth in a small-sized SWG are very small, tooth root stress in FS cannot been measured with strain gauges. Therefore, in this study, strain gauges were pasted on tooth root of Circular Spline (CS) which has thickness rim in the large-sized SWG and tooth root stress in CS was measured. Next, influence coefficients of CS between tooth root stress and load on tooth surface were derived. With these result, load distribution on tooth surface was easily calculated. Distribution of tooth root stress in FS was calculated by using FEM.