In this study, self-loosening and fatigue tests of bolt and nut joints subjected to transverse vibrations have been performed to understand the loosening-fatigue characteristics. The current study is especially focused on the influences of lubricant of the bolt-nut joint on the loosening-fatigue characteristics. The result shows that fatigue failure of the bolt-nut joint occures due to propagation of fatigue crack. The result also shows that a lubricant influence on the loosening-fatigue characteristic under transverse vibration.