Microcirculation plays an important role in mass transport in human cardiovascular system, it is important to understand blood flow in microvessels. In such small channels, red blood cell behavior strongly affects the flow field. In this study, we investigate in vitro a blood flow through a microchannel with stenosis by confocal-micro PIV and PTV. The flow channel is made by PDMS (Polydimethylsiloxane) channel and the constriction rate of the stenosis is 50%. We measure the blood flow velocity, red blood cell trajectories and plasma layer. The results show that the blood flow has small flactuations and is different from water.