This paper presents a three-dimensional PTV technique for the flow around a rotating object. The present technique is based on a defocusing method of integral photography for three-dimensional measurement and a derotating observation with an image-rotation prism for measuring the flow relative to a rotating object. In the former method, particle images are captured using a multi-pinhole aperture set in front of a camera lens, and then the images are numerically reconstructed with a virtual pinhole aperture. Experimental results show that the particle images on a rotating disk are successfully derotated using the present technique and the correlation between true particle depth and the image location detected in integral photography can be calibrated with small deviation.