Nanometer size particulate matter (Nano-PM) emitted from a flame of fossil fuel has been received attention due to their harmful potential for health. However, detailed knowledge about Nano-PM formation mechanism has not been clarified yet. The objective of this study is to investigate the relation between the PM characteristics and sulfur in fuel using standard Nano-PM generator. A pool flame was used for production of Nano-PM. Kerosene and Thiophen added Kerosene were used as fuels. It was clear that the flame form changed from closed flame to open flame, when fuel flow rate M_f was over 0.4mg/s in the both fuels. The flame tip of open flame was opened and visible soot was emitted from the tip. As for the size distribution of PM, the peak diameter was gradually increased with an increase of fuel flow rate in Kerosene flame, but it was suddenly increased when the fuel flow rate exceeded 0.4mg/s, in Thiophene added Kerosene flame.