748 Adhesion of a Single Chondrocyte on RGDS-transgenic Fibroin Substrate
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RGDS-transgenic fibroin scaffold have been expected to be one of the useful materials for cartilage regeneration where chondrocytes can synthesize matrix to improve lubricant function. For the purpose of seeking some aspects of the mechanism of interactions between a single chondrocyte and RGDS-transgenic fibroin substrate, adhesive force of a chondrocyte to the substrate was measured. It was found that the adhesive force of a chondrocyte on RGDS-transgenic fibroin substrate was higher than that on normal fibroin substrate between 3 and 12 h after seeding, while significant differences were not shown in cell spreading area. It could be considered that integrin expression and/or its distribution affected the adhesive force. Immunofluorescence staining for actin and vinculin showed that the cell morphology could be influenced by integrin-mediated signaling changes. These results suggested that initial cell-material adhesion have an important role to affect the formation of functional cartilage tissue.
