This study was aimed to determine in vivo kneeling kinematics after total knee arthroplasty (TKA). Image matching technique with high-resolutional flat panel detector (FPD) was used for the motion analysis of kneeling and walking. We focused on relative location between the femoral component and polyethylene insert. The result indicated that motion analysis of artificial knee implants could clarify typical motion patterns depending on patient. Our analysis could reproduce in vivo relation of contact between a femoral component and polyethylene insert on a computer, which gave us important information that could not be obtained by conventional X-ray image.