Fatigue crack growth test was carried out on α-brass. Successive observation of small fatigue crack growth behavior was performed by means of an Atomic Force Microscope (AFM) equipped with small in-plane bending fatigue testing machine. The fatigue crack almost propagates by using preferred slip system. Occasionally, the crack propagates by using other slip systems. So, the fatigue crack propagates with deflections repeatedly. The fatigue crack deflection behavior was investigated by the crystallographic orientation analysis based on the Electron Backscatter Diffraction (EBSD). Furthermore, in order to examine internal crack surface feature, the crack pass of specimen that was etched by Focused Ion Beam (FIB) was observed by SEM. It is possible to explain the mechanism of fatigue crack behavior mentioned above by using Slip factor and examining the relationship between the inclination of internal fatigue crack plane and the crystal orientation.