This paper deals with the assessment of low cycle fatigue life under intermittent strain amplitude for Incone1718. Several combinations of intermittent strain fatigue tests were conducted. Intermittent waveform includes not only major cycle but also minor cycle with mean stress. The Walker equation based on virtual elastic stress was used in this study to consider the mean stress effect on minor cycle. The shortest fatigue life was about 1/10 of estimated one without using Walker stress. On the other hand, the predicted lives based on Walker stress were within a factor of 3 scatter band. Therefore, fatigue life estimation for intermittent cycle should be considered mean stress effect on minor cycle damage.