The purpose of this study is to engineer skeletal muscle tissue from myoblasts for development of bio-actuator. We evaluated the contractile force of the complex tissue made of elastase treated tissue and collagen gel as scaffold for engineered skeletal muscle. The C2C12 cells were seeded in collagen gel and cultured at 37℃ under 5% CO_2. After 10 days in culture, tissue-engineered muscle contracted with electrical pulse stimuli and, the isometric contractile force was 900 μN at 1 Hz. Hematoxylin-eosin staining showed that myoblasts remained inside the collagen gel and differented to the myotube.
