Although the number of the patients with skin cancer has increased every year. noninvasive diagnosis methods of skin cancer have not been established yet. It is reported that Catehpsin L (CatL) correlated with actinic keratosis and squamous cell cancer. In this study, an novel immuno-chromatographic test-strip is proposed for Cad, analysis in skin. The fabricated test-strip consists of four pads, a sample pad. a conjugate pad, a nitrocellulose membrane and a absorption pad. After dropping a sample. it required 90 seconds to reach the solution at the test line on the test-strip and indicated a red colored band 3 minutes later. We proposed that this newly developed immuno-chromatographic test-strip has a highly potential as a tool for early diagnosis of skin cancer.