It is clarified that when endothelial cell covering over aortic valve is damaged, the aortic valve is hardened, which is known as aortic valve sclerosis. However, it is almost unknown that how and why endothelial cells are damaged. In this study, realistic models of aortic leaflets and aortic root were made, and the shear stress and normal stress acting on the model leaflet was measured by stereo PIV and observation of model leaflet. As a result, it is found that the shear stress and normal stress act along the model leaflet in the vicinity of its root in a diastole, and it was suggested that these stresses caused by blood flow were factors of the endothelial cell damages.