The microcapsules for medical application such as the drug carrier in the Drug Delivery System and the simulated red blood cell in the model blood in vitro are actively researched. In this study, we produced the microcapsule with three-layer thin film of alginate-poly-L-lysine-alginate based on alginate gel particles. We examined the membrane thickness and elastic property. As a result, increase of incubation time with poly-L-lysine induces increase of the membrane thickness and the elasticity of the microcapsule.