A new PVTt system has been developed to establish the national standard for a gas flowrate of less than 5 mg/min. It, is presented in this paper that a mass of gas gathered in the constant volume tank is the most important parameter on an uncertainty analysis of a PVTt system and the uncertainty of a flowrate must be described with the collection time. This PVTt system can calibrate a flow meter with a desired uncertainty for the flowrate range less than 5 mg/min, because it is possible to calibrate a test flow meter over several days without disturbances of the upstream condition.