Recently, motor protein such as kinesin or dynein has been used as a nano actuator. Previous works have achieved the control of the movement of microtubules (MT) and the transport of molecular cargo. However, the movement of microtubules was led by minus-end because only kinesin was used as an actuator. In this paper, we patterned motor proteins selectively by surface modification and control the location of microtubules by electrophoresis. Selective patterning of motor proteins and actively control of the MT location enables MTs to move with minus-end and plus-end as leading heads by kinesin and dynein motors.