T0201-1-6 Study on the role of actomyosin contractility in lamellipodial protrusion dynamics using a micropattered substrate

Abstract

Actin polymerization-driven protrusion of the lamellipodia is a requisite step during cell migration. A cell interacts with the extracellular matrix and is affected by mechanical properties of their physical environment. The effect of actomyosin contractility generated from the interactions involving myosin II in cell locomotion is less investigated. This study focuses on the effects of the actomyosin activation on cell protrusion dynamics utilizing micropattern which has both adhesive region and adhesion suppressed region. Our results reveal that up-regulating actomyosin activity leads to an increase in lamellipodial length and increased migration across the gaps.