This report describes a newly developed cascade impacter pollen collection device. We performed an adhesion experiment using alder pollen, and found the results to be in close agreement with the results predicted by a numerical analysis based on the Navier-Stokes equation and the Euler-Lagrange method. Based on this airflow method, we analyzed the separation of four typical Japanese pollens under various conditions of air suction velocity and nozzle diameter. Pollen grains are collected in four stages of the device. To separate pollens adhered to the same plate, we used an automatic pollen measuring system, which allowed pollen grains to be completely separated.