Abstract
This paper discusses an approach to prepare bilayer lipid membrane (BLM) using droplet contact method at micro-scale. To substantialize this method, Split-and-Contact Device (SCD) which makes the BLM by mechanical motion was developed. A method using BLM are known as a powerful tool for the ion channel analysis or membrane protein study. Micro SCD is a device that applied the SCD concept and miniaturized well opening length concept, result to micro-scale droplet interface region. When the droplets are split and joined together, the lipid monolayer surrounding them at the interval combine to form a robust lipid bilayer. The α-hemolysin (αHL) that acts as pores, incorporate into the lipid bilayer, allows the ionic currents to be flow across lipid bilayer, and measured. Here, we present three different well opening size that are 500, 300 and 150 μrn. This system was able to fabricate stable BLM and the low current noise recordings of aHL channel were achieves using this system.
