Deformation of liposomes using microstructures formed by multiphoton lithography

Abstract

We created the non-spherical liposomes with artificial micro-cytoskeleton. First, we fabricated micro-cubic frames with 3D-laser-printed photoresists. Next, we formed water-oil-water interface on the faces of these microstructures in a microfluidic channel. Finally, we confirmed whether this interface was lipid bilayer membrane by using α-hemolysin as a membrane protein. As a result, we successfully observed the solution encapsulated by lipid bilayer membrane formed around the microstructures.