Abstract
This paper describes an evaluation of the effect of three-dimensional (3D) tissue shape on differentiation ratio and spatial distribution of neurons. We controlled the 3D tissue shape by culturing neural stem cells in a closed agarose microchamber and induced differentiation of neural tissues with various 3D shapes. By measuring the neuron ratio in the neural tissues, we confirmed that thinner tissues have higher neuron ratio and more uniform distribution of neurons. We believe that our culture method could fabricate neural tissues of high neuron ratio and uniform distribution of neurons and be application to drug screening of 3D neural tissues.
