Abstract
This paper reports a method of alginate hydrogel module assembly for heterogeneous 3D structure. This assembly was done by connecting alginate gel modules with ‘cationic nanoparticle’ (CNP) solution. The adhesion is caused by the interaction between CNP and the anionic surface of alginate gel modules. We showed that our method can connect alginate gel modules without external stimuli or maleffect to alginate gel modules with human malignant epithelial cells (HeLa). Alginate gel plates were connected with CNPs and its connection was confirmed to be kept under a mechanical stress. As a demonstration, we achieved to make a bundle of microfiber containing HeLa cells. We believe that our method provides a suitable method for fabrication of artificial tissue structures in the field of tissue engineering.
