G103 Time-Series Microscopic Behavior of Ice Crystals during Recrystallization in Frozen Biological Tissues

Abstract

Behavior of ice crystals and cells in the biological tissues during the slow-warming after rapid-cooling was investigated microscopically on real time using a confocal laser scanning microscope with a fluorescent dye. Attention was paid on the recrystallization of intracellular ice crystals. The recrystallization could increase the mechanical damage of the tissues due to ice crystals. Size and number of the ice crystals were measured from the image-data of ice crystals. The size of ice crystals was statistically analyzed. Frequency, average, standard deviation, sum of amount of ice crystals, etc. were made clear in time-series during the slow-warming.