This paper describes motion analysis of a lower limb movement during a pedaling movement of a cyclist with the iliotibial band syndrome. The cyclist's lower limb movement is analyzed by a three dimensional motion analysis system. In this study, two sets of experiments are conducted, in which the subject pedals on a cycle against a load with or without a cleat wedge on the cycling shoes. The experimental results show that the magnitude of the movement of the patella of the affected side is significantly larger than that of the unaffected side. Furthermore, it is found that the cycling shoes with adequate cleat wedge decrease the magnitude of the movement of the patella in the coronal axis.