The aim of this study was to determine the influence of pull-up action on the pedaling mechanics and muscle coordination during short term cycling. Eight male cyclists performed three bouts of cycling for 30 sec on different pedaling images; preferred, emphasizing pull-up action and push-down. Each exercise bout is consisted of the onset of cycling for 10 sec, a constant load cycling for 10 sec and a maximal effort for 10 sec. Pedal force, crank force, crank revolution and EMG signals from leg muscle were measured. A significant difference was observed in the index of force effectiveness (IFE) at constant load exercise phase between pull up images and other two conditions. However, clear differences were not seen in the EMG.