Abstract
A simultaneous measurement technique for velocity and pH distributions was developed by using fluorescence and a confocal scanner. The present study examines the ability to combine micro-PIV using fluorescent particles and laser induced fluorescence (LIF) with Fluorescein sodium dye whose intensity is strongly dependent on pH. A prism inside a 3CCD camera separates particles' fluorescence from that of dye. Two buffer solutions with different pH values were introduced through a T-shaped microchannel. Velocity-vector maps were obtained by ensemble-averaging velocities of instantaneous images with the spatial resolution of 15μm×15μm. A two-dimensional distribution for the flow field with a pH gradient was detected from fluorescent intensities with the spatial resolution of 5μm×5μm.
