Abstract
Cell damage and death due to the hypertonic electrolyte solution under the unfrozen condition were investigated experimentally. The cells were exposed from an isotonic solution to hypertonic electrolyte solution (concentration C_m and exposure time τ) back to the isotonic solution. The membrane integrity was assessed by means of the dye exclusion test to estimate the cell viability. Influence of temperature on the cell viability as a function of τ and C_m was clarified. The results indicate that the cell damage and death proceed kinetically in the similar way. Also, an interesting dependency of the cell viability on temperature was found out.
