Abstract
Fluorescence image was investigated for development of a leukocyte counter for quality assurance tests of transfusion blood products. The standard leukocyte fluorescence image profile was established by averaging the actual fluorescence image of leukocytes. It was used for development of an optimum image analysis algorithm to avoid miss-counting of the dust contaminated from the environment. The centrifugal concentration process was investigated by a computer simulation, and it was found that this process caused leukocyte overlapping and limited the measurement range. We succeed in developing the leukocyte counter, with improving the accuracy and the measurement range up to 25 [WBC/μl].
