Abstract
We observe the gradual, one-directional freezing procedure of the aqueous solution of HPLC6, which is the major fraction of the antifreeze protein type I. The location of the protein is obtained from the fluorescence tagged to the protein. It is observed that the proteins slide on the interface. Also, long, narrow liquid regions are formed inside the ice from the depression parts due to the highly associated proteins. It is found from our supplementary computation that these liquid regions can be predicted.