Abstract
We have carried out an experiment on the freezing aqueous solution flow of winter flounder antifreeze protein in a micro-channel of 0.15mm in height, 1.2mm in width and 21mm in length. An electro-osmotic pump was used to create the flow of the solution in the channel. The flow was cooled from the side wall. An ice/solution interface is observed at low magnification in a high-resolution biological microscope. It is found from our experiment that, as the flow rate increases, the growing rate of the ice/solution interface decreases and the interface becomes more serrated. This is because the interaction between the interface and the protein, continuously approaching the interface due to the flow, occurs more frequently with an increase in the flow rate.