Abstract
This paper describes a method for perforating fish egg (Medaka) membrane efficiently by using a rectangular electric pulse applied between the plate electrode and the hollow needle electrode. Minimum required pulse strength to perforate egg membrane (chorion and yolk membrane) was measured under the various pulse lengths and concentrations of supporting liquid. The results ahowed that the minimum required pulse strength was decreased with increasing pulse length and electric conductivity of the solution. In addition, egg membreanes in the hypertonic solution (〜130mOsm/kg) could not be perforated because of their severe deformation. It can be concluded that appropriate high electric conductivity avoiding egg's doformation might be required to perforate fish membrane with low pulse strength.
