Abstract
A method that can measure the fluid temperature in microscopic scale by measuring the fluorescence polarization is described in this study. To understand the basic characteristics and advantage of the method, the effects of the fluid temperature, fluid viscosity, measurement time, and pH of the solution on the measured fluorescence polarization degree are discussed. The results showed that fluorescence polarization is considerably less sensible to the measurement time and pH compared to the fluorescence intensity demonstrating the stability of the temperature measurement. The correlations between the fluid temperature and the inverse value of P measured by two photo detectors (photomultiplier and CCD camera) both showed a linear relationship. This agreed with the theoretical characteristic, and confirmed the feasibility of this method.
