Abstract
Biosensors are important measurement tools for diagnosis and drug discovery. Especially in drug-discovery processes, high-throughput biosensors are required because of many drug candidates. Then we developed a new concept high-throughput biosensor using picosecond ultrasound method. A metal microdot is irradiated with ultrashort optical pulse, where ultrahigh-frequency phonons are excited. They are transferred into the solution across the proteins captured on the microdot, leading to the phonon intensity change. We observe this phenomenon using a time-resolved pump-probe method. We can detect 67 pM rabbit IgG using reflective change caused by phonon intensity change due to protein coupling onto the sensor chip. In addition, we achieved detection of difference between specific binding and non-specific binding using multi-channel microdots. And we developed theoretical calculation for this measurement principle. In this study, Our new concept biosensor can be a powerful tool as a large-scale multichannel biosensor.
