Detection of water content distribution and phase change in bio tissue using near-infrared

Abstract

In order to enable high quality cryopreservation of foods and bio tissue, it is necessary to consider the effect of the texture on the formation and melting of ice crystals in the cell and tissue. In addition to the above, it is necessary to clarify the process of melting and regrowth of ice crystals in bio tissue during cryopreservation and control the particle size of ice crystals. In this study, we attempted to observe the internal state of biological samples non-destructively by irradiating a sample with near infrared light, which has high body permeability, and detecting the distribution of water and ice crystals in bio tissue by utilizing the difference in the near-infrared transmittance to blood, lipids, water, and ice.