Effect of temperature and the stop of active transport on cell damage by cold storage.

Abstract

We attempted to discriminate active-transport stop by measuring electrical impedance at the ambient temperature that seemed to be the boundary of active-transport stop in cold stored cells, and compared it with the survival rate after storage. Rat cardiac myocyte was stored at 4 to 7°C for 3 to 12 h, and the electric impedance was measured during storage. The results showed that the electric impedance of rat cardiac myocyte was significantly lower at 4°C and 5°C for 12 hours than at 3 hours, and the survival rate was also lower. It was suggested that influence of an active-transport stop on cell damage was great.